DMK Valproate and Saccharin CTA 2

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Repeat of DMJ Valproate and Saccharin CTA.

Data

Raw Data Link

People

Durba Mukherjee

CTA Conditioning

Agenda

Rats arrive ( July 6, 2021) n=16 adult male (8) and female(10) sprague-dawley rats (Charles River CD), individually housed in BRF 169.


Day 0: Water removed ( July 14, 2021)


Days 1 - 7: Water Restriction ( July 15 - 21 , 2021)

Weigh rats daily. Water for 30 min, ad lib food.

30 min water at 1430 daily.


Day 8: Conditioning Day (July 22 2021)

-15 min: half of rats injected with 0.15M Na Valproate 8ml/kg = 200mg/kg; other half 0.15M NaCl 8ml/kg 0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. 40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip.

ad lib water returned after final injection overnight.

  • rats 1-8 : male, Rats 9-16: female
  • rats 1-4, 9-12: Valproate; rats 5-8,13-16 NaCl
  • All rats: saccharin
  • odd rats: 3ml/kg of 0.15M LiCl; odd rats: 3ml/kg of 0.15M NaCl


Days 9 - 20: Preference Tests (July 23 - August 4, 2021)

Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 12 days.