DMS Valproate 100mg/kg Repeat
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Repeat of Valproate-enhanced LiCl-CTA in male and female rats.
| Valproate 100 mg/kg Enhanced CTA pt2 | |
|---|---|
| HDAC Sex Difference | |
| Expt Code | DMS |
| Start Date | 2022-1-15 |
| Investigators | Mukherjee, Houpt |
| Funding | Chair's Fund |
| Protocol | 1917 |
| Expt Type | CTA |
| Species | rat |
| Drugs | valproate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMS |
Data
People
Durba Mukherjee
Agenda
Rats arrive (2202-1-11)
Sprague Dawley rats (dob males: 2021-11-15; females: 2021-10-25 )
Day 0: Water removed 2022-1-15
Days 1-8: Water Restriction 2022-1-16 thru 2022-1-23
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
Day 9: Conditioning Day 2022-24
- -15 min: 8 ml/kg (100 mg/kg) of Valproate 75mM
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg ip of 0.15M LiCl or NaCl
Groups:
- DMS01-04 Val-Li-Male
- DMS05-08 Val-NaCl-Male
- DMS09-12 Val-Li-Female
- DMS13-16 Val-NaCl-Female
ad lib water returned after final injection overnight.
Days 10-21: Preference Tests: 2022-1-25 to 2022-2-6
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 12 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.