SNA Lithium Orotate CTA
Test if lithium orotate (LiOr) induces a conditioned taste aversion comparable to LiCl.
Single pairing of saccharin intake (15 min) after 15-min delay with isomolar doses of LiCl and LiOr to see if LiOr is more potent.
| Lithium Orotate and CTA pt1 | |
|---|---|
| Taste Aversion | |
| Expt Code | SNA |
| Start Date | 2025-1-14 |
| Investigators | Nabi, Houpt |
| Funding | Chair's Fund |
| Protocol | 20220000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | lithium orotate, lithium chloride |
| Data Link | https://houptlab.org/bartab/expt/?id=SNA |
Data
People
Soheila Nabi
CTA Conditioning
Agenda
Rats arrive (2026-1-20), n= 16 adult male Sprague Dawley rats (Charles River CD), individually housed in BRF 169.
Day 0: Water removed (2026-1-26)
make labels for bottles.
Days 1 - 6: Water Restriction (2026-1-27 thru 2026-2-1)
Weigh rats daily. ad lib food.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 1400 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
Day 12: Conditioning Day (2026-2-2)
0 min: 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min: rats injected with:
- 0.15 M NaCl, 6 ml/kg ip. (SNA01,04,07,10,13)
- 0.15 M LiCl, 6 ml/kg ip. (SNA02,05,08,11,14)
- 0.15 M LiOr, 6 ml/kg ip. (SNA03,06,09,12,15,16) (LiOr was more of a milky suspension)
ad lib water returned after final injection overnight.
Days 13 - 22: Preference Tests (2026-2-3 through 2026-2-13)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.