DMA Phosphoacetylated Histones after Flavor Conditioning

People

Durba Mukherjee, T.A. Houpt

Procedure

Sprague Dawley rats (male to begin with) singly housed with ad lib access to food and water. Habituated to a food and water restriction schedule of 30 min water and food, then 4.5 hours of food alone, every day for 7 days. On conditioning/perfusion day, rats given 30-min access to 1 of 3 solutions:

• water
• 0.05% saccharin + 0.05% Kool-aid (Grape and Cherry counterbalanced)
• 8% Glucose + 0.05% saccharin + 0.05% Kool-aid (Grape and Cherry counterbalanced).

Rats perfused at different time points after end of access and brains processed for phospho-acetylated histones (need to add specific antibody).

Time Line

• 2019-June-4 Rats (n=9) arrive
• 2019-June-5 Remove food and water
• 2019-June-6 30 min water at 1100-1130, 5 hour food 1100-1600 (Day 1)
• 2019-June-7 30 min water, 5 hour food (Day 2)
• 2019-June-8 30 min water, 5 hour food (Day 3)
• 2019-June-9 30 min water, 5 hour food (Day 4)
• 2019-June-10 30 min water, 5 hour food (Day 5)
• 2019-June-11 30 min water, 5 hour food (Day 6)
• 2019-June-12 30 min water, 5 hour food (Day 7)
• 2019-June-13 30 min water or kool-aid or kool-aid + glucose (n=3/group); perfuse 1 hour later