View source for DMG Sodium Butyrate and Saccharin-LiCl CTA

Replication (to increase N) of [[DMF Sodium Butyrate and Saccharin-LiCl CTA]]

Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .

A more strict replication of [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962].

1. Ordered brand new Sodium Butyrate ([https://www.sigmaaldrich.com/catalog/search?term=303410	Sigma-Aldrich 303410-100G] Butyric acid sodium salt, 98% ).

2. n=8 male, n=8 female, counterbalanced


Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] 

* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)

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==Data== 

'''[https://houptlab.org/bartab/expt/?id=DMG Raw Data Link]'''

==People== 

Durba Mukherjee


==CTA Conditioning==

===Agenda===

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'''Rats arrive''' (November 3, 2020)
n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.

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'''Day 0: Water removed''' (November 9, 2020)

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'''Days 1 - 11: Water Restriction''' (November 10 - November 17, 2020)

Weigh rats daily.  water for 10 min, ad lib food. 

10 min water at 1430 daily.

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'''Day 12: Conditioning Day'''  (November 18, 2020)

0 min: 30 min access to 0.125% saccharin   (5 g sodium saccharin/4L water)
30 min: all rats injected with 0.15 M LiCl, 3 ml/kg ip.
40 min:  half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip

ad lib water returned after final injection overnight.

* Rats 1-8 : female, Rats 9-16: male
* All rats: saccharin
* All rats: LiCl (0.15M, 3ml/kg)
* rats 1-4, 9-12:  NaCL injection; rats 5-8,13-16: NaB injection; 


'''Days 13 - ??: Preference Tests''' (November 19 - ?? November  , 2020)

Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.


[[Category:Flavor Preference]]
[[Category:Taste Aversion]]