View source for Methylation-Sensitive Enzymes


Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site.

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Enzyme	NEB link	Site	Cut Position	Reaction Conditions
HpaII	https://www.neb.com/products/r0171-hpaii	CCgg	5’...C/CGG...3'<br>3’...GGC/C...5’	1X NEBuffer 1, Incubate at 37°C.
MspI	https://www.neb.com/products/r0106-mspi	CCgg	5’...C/CGG...3'<br>3’...GGC/C...5’	1X NEBuffer 4, Incubate at 37°C.
AciI	https://www.neb.com/products/r0551-aciip	CCgC	5’...C/CGC...3'<br>3’...GGC/G...5’	1X NEBuffer 3, Incubate at 37°C.
HhaI	https://www.neb.com/products/r0139-hhai	gCgC	5’...GCG/C...3'<br>3’...C/GCG...5’	1X NEBuffer 4, Supplemented with 100 μg/ml Bovine Serum Albumin, Incubate at 37°C.
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[[Category:Protocols]]