Methylation-Sensitive Enzymes

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Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site.

Enzyme NEB link Site Cut Position Reaction Conditions
HpaII https://www.neb.com/products/r0171-hpaii CCgg 5’...C/CGG...3'
3’...GGC/C...5’
1X NEBuffer 1, Incubate at 37°C.
MspI https://www.neb.com/products/r0106-mspi CCgg 5’...C/CGG...3'
3’...GGC/C...5’
1X NEBuffer 4, Incubate at 37°C.
AciI https://www.neb.com/products/r0551-aciip CCgC 5’...C/CGC...3'
3’...GGC/G...5’
1X NEBuffer 3, Incubate at 37°C.
HhaI https://www.neb.com/products/r0139-hhai gCgC 5’...GCG/C...3'
3’...C/GCG...5’
1X NEBuffer 4, Supplemented with 100 μg/ml Bovine Serum Albumin, Incubate at 37°C.