NMDA Receptors

Primers for Davenport's Real-Time PCR experiment

Primers were ordered 2009 March from Sigma Aldrich

Probe Abbr Sequence Tm Size Genbank Reference
NR1 RTNR1FOR GTTCTTCCGCTCAGGCTTTG 66.0 66 RNU11418, 2396-2461 Yamamoto et al. 2008 PMID 17957211
RTNR1REV AGGGAAACGTTCTGCTTCCA 65.8
Serine Racemase RTSERRACFOR ATTGCAAGAAACTGGCCATC 63.9 295 NM198757, 664-958 Yoshikawa et al., 2006 PMID 16973158
RTSERRACREV TCAGCAGCGTACACCTTCAC 64.1
GADPH RTGADPHFOR TGCACCACCAACTGCTTAG 62.5 201 DQ403053.1, 397-597 Uno et al., 2002 PMID 12068078
RTGADPHREV GGATGCAGGGATGATGTTC 63.0
Note: GADPHFOR and REV maybe mixed up . . . .

Primers for Real-Time PCR

Taken from Yamamoto et al. 2008 PMID 17957211 using Applied Biosystems

NR1

Forward primer	5'-GTTCTTCCGCTCAGGCTTTG-3'
Reverse primer	5'-AGGGAAACGTTCTGCTTCCA-3'
TaqMan probe	5'-FAM-CGGCATGCGCAAGGACAGCC-TAMRA-3'
	 

NR2A

Forward primer	5'-AGCCCCCTTCGTCATCGTA-3'
Reverse primer	5'-GACAGGGCACCGTGTTCCT-3'
TaqMan probe	5'-FAM-AGGACATAGACCCCCTGACTGAGACCTGTG-TAMRA-3'
	 

NR2B

Forward primer	5'-CCCCCAAGTTCTGGTTGGT-3'
Reverse primer	5'-TTTTGGGAACGAGCTTTGCT-3'
TaqMan probe	5'-FAM-TTGGCCGTCTTGGCCGTATCAGG-TAMRA-3'
	 

NR2C

Forward primer	5'-GGGTGATGATGTTCGTGATGTG-3'
Reverse primer	5'-GTGAGGTTCTGGTTGTAGCTGACA-3'
TaqMan probe	5'-FAM-TCACGGTGGTTGCCATCACTGTCTTC-TAMRA-3'

Primers chosen for experiment (according to Andre's advice)

(designed using PrimerPlus3

NR1 U11418.1 2360 Forward ttcacagaagtgcgatctgg, Tm 60.0 2467 Reverse atggacagggaaacgttctg, Tm 60.0 Product size of 108 bp

Serine Racemase NM_198757

856 forward: tggtagatgcgttggtggta, Tm 60.0 958 reverse: tcagcagcgtacaccttcac, Tm60.1

674-807, product size 134bp

Rat GAPDH

Forward TCACTGCCACTCAGAAGACTG Reverse GGATGACCTTGCCCACAGC