Difference between revisions of "DMK Valproate and Saccharin CTA 2"
(created page) |
(added received date) |
||
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
+ | Repeat of [[DMJ Valproate and Saccharin CTA]]. | ||
+ | |||
+ | ==Data== | ||
+ | |||
+ | [https://houptlab.org/bartab/expt/?id=DMK Raw Data Link] | ||
+ | |||
+ | ==People== | ||
+ | |||
+ | Durba Mukherjee | ||
+ | |||
+ | ==CTA Conditioning== | ||
+ | |||
+ | ===Agenda=== | ||
+ | |||
+ | '''Rats arrive''' ( July 6, 2021) | ||
+ | n=16 adult male (8) and female(10) sprague-dawley rats (Charles River CD), individually housed in BRF 169. | ||
+ | |||
+ | <br> | ||
+ | |||
+ | '''Day 0: Water removed''' ( July 14, 2021) | ||
+ | |||
+ | <br> | ||
+ | |||
+ | '''Days 1 - 7: Water Restriction''' ( July 15 - 21 , 2021) | ||
+ | |||
+ | Weigh rats daily. Water for 30 min, ad lib food. | ||
+ | |||
+ | 30 min water at 1430 daily. | ||
+ | |||
+ | <br> | ||
+ | |||
+ | '''Day 8: Conditioning Day''' (July 22 2021) | ||
+ | |||
+ | -15 min: half of rats injected with 0.15M Na Valproate 8ml/kg = 200mg/kg; other half 0.15M NaCl 8ml/kg | ||
+ | 0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) | ||
+ | 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. | ||
+ | 40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip. | ||
+ | |||
+ | ad lib water returned after final injection overnight. | ||
+ | |||
+ | * rats 1-8 : male, Rats 9-16: female | ||
+ | * rats 1-4, 9-12: Valproate; rats 5-8,13-16 NaCl | ||
+ | * All rats: saccharin | ||
+ | * odd rats: 3ml/kg of 0.15M LiCl; odd rats: 3ml/kg of 0.15M NaCl | ||
+ | |||
+ | |||
+ | '''Days 9 - 20: Preference Tests''' (July 23 - August 4, 2021) | ||
+ | |||
+ | Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 12 days. | ||
+ | |||
+ | |||
+ | [[Category:Flavor Preference]] | ||
+ | [[Category:Taste Aversion]] |
Latest revision as of 12:28, 26 August 2021
Repeat of DMJ Valproate and Saccharin CTA.
Data
People
Durba Mukherjee
CTA Conditioning
Agenda
Rats arrive ( July 6, 2021) n=16 adult male (8) and female(10) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
Day 0: Water removed ( July 14, 2021)
Days 1 - 7: Water Restriction ( July 15 - 21 , 2021)
Weigh rats daily. Water for 30 min, ad lib food.
30 min water at 1430 daily.
Day 8: Conditioning Day (July 22 2021)
-15 min: half of rats injected with 0.15M Na Valproate 8ml/kg = 200mg/kg; other half 0.15M NaCl 8ml/kg 0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. 40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip.
ad lib water returned after final injection overnight.
- rats 1-8 : male, Rats 9-16: female
- rats 1-4, 9-12: Valproate; rats 5-8,13-16 NaCl
- All rats: saccharin
- odd rats: 3ml/kg of 0.15M LiCl; odd rats: 3ml/kg of 0.15M NaCl
Days 9 - 20: Preference Tests (July 23 - August 4, 2021)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 12 days.