Difference between revisions of "JB Systemic Sodium Selenate"
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Rats (n= 24) on 8 days of water restriction (3h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min). ''Start Restriction on Tuesday, 2011-11-29'' | Rats (n= 24) on 8 days of water restriction (3h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min). ''Start Restriction on Tuesday, 2011-11-29'' | ||
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| + | Weigh rats ''Wednesday, 2011-12-7'' | ||
Conditioning day ''Thursday, 2011-12-8'': | Conditioning day ''Thursday, 2011-12-8'': | ||
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at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12) | at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12) | ||
| + | :For isotonic solution of sodium selenate: Na2Se04 at 12 mg/ml (63 mM), NaCl at 3.2 mg/ml (55mM) = 300 mOsm | ||
at 0 h, 10 min access to 0.125% saccharin (CS) | at 0 h, 10 min access to 0.125% saccharin (CS) | ||
| Line 22: | Line 25: | ||
Next day start 2-bottle, 24-h preference test (saccharin vs. water) for 14 days. ''End on Friday, 2011-12-23.'' | Next day start 2-bottle, 24-h preference test (saccharin vs. water) for 14 days. ''End on Friday, 2011-12-23.'' | ||
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| + | Results: While NaCl-LiCl rats acquired a moderate aversion, the dose of sodium selenate was too high: all of the selenate treated rats acquired a strong CTA, with zero preference for saccharin that persisted for all 14 days of 2-bottle testing. | ||
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| + | Conclusion: next time, we'll reduce dose of sodium selenate. | ||
'''2. Test c-Fos induction''' | '''2. Test c-Fos induction''' | ||
| − | + | 2012-1-10 perfused JB 1-5 | |
| + | |||
| + | 2012-3-14 perfuse JB 6-10 | ||
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| + | Reuse same rats, shuffled into new groups: | ||
| + | |||
| + | at -2 h, Inject with sodium selenate in NaCl 0.15 M , 1 ml/kg at 4 doses: | ||
| − | + | *0 mg/kg Na2Se04 | |
| + | *0.5 mg/kg Na2Se04 | ||
| + | *1 mg/kg Na2Se04 | ||
| + | *2 mg/kg Na2Se04 | ||
| + | |||
| + | and 0.15M LiCl 12 ml/kg as a positive control. | ||
| − | |||
| − | at | + | at 0 h, perfuse rats. |
process for c-Fos (~ 1 week in the lab) | process for c-Fos (~ 1 week in the lab) | ||
| + | |||
| + | [[JC & JD Systemic Sodium Selenate]] | ||
| + | |||
| + | [[Category: CTA Experiments]] | ||
| + | [[Category:Taste Aversion]] | ||
Latest revision as of 07:48, 26 May 2023
(Brief background of the experiment).
Sodium Selenate (Na2Se04) activates PP2A.
Agenda
1. Test Conditioned Taste Aversion (23 days)
Rats (n= 24) on 8 days of water restriction (3h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min). Start Restriction on Tuesday, 2011-11-29
Weigh rats Wednesday, 2011-12-7
Conditioning day Thursday, 2011-12-8:
4 groups (n=6 per group) : NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl
at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)
- For isotonic solution of sodium selenate: Na2Se04 at 12 mg/ml (63 mM), NaCl at 3.2 mg/ml (55mM) = 300 mOsm
at 0 h, 10 min access to 0.125% saccharin (CS)
at 0.5 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)
at 1.5 h, Return water overnight
Next day start 2-bottle, 24-h preference test (saccharin vs. water) for 14 days. End on Friday, 2011-12-23.
Results: While NaCl-LiCl rats acquired a moderate aversion, the dose of sodium selenate was too high: all of the selenate treated rats acquired a strong CTA, with zero preference for saccharin that persisted for all 14 days of 2-bottle testing.
Conclusion: next time, we'll reduce dose of sodium selenate.
2. Test c-Fos induction
2012-1-10 perfused JB 1-5
2012-3-14 perfuse JB 6-10
Reuse same rats, shuffled into new groups:
at -2 h, Inject with sodium selenate in NaCl 0.15 M , 1 ml/kg at 4 doses:
- 0 mg/kg Na2Se04
- 0.5 mg/kg Na2Se04
- 1 mg/kg Na2Se04
- 2 mg/kg Na2Se04
and 0.15M LiCl 12 ml/kg as a positive control.
at 0 h, perfuse rats.
process for c-Fos (~ 1 week in the lab)