Difference between revisions of "Fluorescent Microscopy"
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| − | Here are the immunofluorescence filters that we probably need. | + | Here are the immunofluorescence filters that we probably need. Bolded are the fluorophores we frequently use. |
<tab sep=tab border=1> | <tab sep=tab border=1> | ||
Probe Excitation (nm) Emission (nm) Color | Probe Excitation (nm) Emission (nm) Color | ||
Neurotrace 435 455 blue | Neurotrace 435 455 blue | ||
| − | DAPI 358 461 blue | + | DAPI 358 461 blue (often use mixed in [https://vectorlabs.com/products/vectashieldr-plus-antifade-mounting-medium-dapi VectaShield]) |
| + | '''DyLight 405''' 400 421 blue | ||
| + | Brilliant Violet 421 407 421 blue (expensive) | ||
| + | '''GFP''' 425 500 green | ||
| + | '''Alexa Fluor 488''' 493 519 green | ||
Fluorescein (FITC) 492 520 green | Fluorescein (FITC) 492 520 green | ||
| + | YFP 500 535 yellow | ||
Cyanine Cy3 550 570 orange | Cyanine Cy3 550 570 orange | ||
| − | Texas Red | + | Alexa Fluor 568 577 602 red |
| − | Cyanine Cy5 | + | Texas Red 589 615 red |
| − | + | '''Alexa Fluor 594''' 591 614 red (sub for Texas Red) | |
| − | + | Cyanine Cy5 650 670 red | |
| + | Alexa Fluor 647 red, not for mercury lamps | ||
</tab> | </tab> | ||
| + | |||
| + | [https://vectorlabs.com/products/vectashield-plus-antifade VECTASHIELD® PLUS Antifade Mounting Medium (H-1900)] | ||
| + | |||
| + | [https://vectorlabs.com/products/vectashieldr-plus-antifade-mounting-medium-dapi VECTASHIELD® PLUS Antifade Mounting Medium with DAPI (H-2000)] | ||
Latest revision as of 15:12, 5 February 2025
Here are the immunofluorescence filters that we probably need. Bolded are the fluorophores we frequently use.
| Probe | Excitation (nm) | Emission (nm) | Color | - |
| Neurotrace | 435 | 455 | blue | |
| DAPI | 358 | 461 | blue (often use mixed in VectaShield) | |
| DyLight 405 | 400 | 421 | blue | |
| Brilliant Violet 421 | 407 | 421 | blue (expensive) | |
| GFP | 425 | 500 | green | |
| Alexa Fluor 488 | 493 | 519 | green | |
| Fluorescein (FITC) | 492 | 520 | green | |
| YFP | 500 | 535 | yellow | |
| Cyanine Cy3 | 550 | 570 | orange | |
| Alexa Fluor 568 | 577 | 602 | red | |
| Texas Red | 589 | 615 | red | |
| Alexa Fluor 594 | 591 | 614 | red (sub for Texas Red) | |
| Cyanine Cy5 | 650 | 670 | red | |
| Alexa Fluor 647 | red, not for mercury lamps |
VECTASHIELD® PLUS Antifade Mounting Medium (H-1900)
VECTASHIELD® PLUS Antifade Mounting Medium with DAPI (H-2000)