Fluorescent Microscopy

Here are the immunofluorescence filters that we probably need. Bolded are the fluorophores we frequently use.

Probe	Excitation (nm)	Emission (nm)	Color
Neurotrace	435	455	blue
DAPI	358	461	blue (often use mixed in VectaShield)
DyLight 405	400	421	blue
Brilliant Violet 421	407	421	blue (expensive)
GFP	425	500	green
Alexa Fluor 488	493	519	green
Fluorescein (FITC)	492	520	green
YFP	500	535	yellow
Cyanine Cy3	550	570	orange
Alexa Fluor 568	577	602	red
Texas Red	589	615	red
Alexa Fluor 594	591	614	red (sub for Texas Red)
Cyanine Cy5	650	670	red
Alexa Fluor 647			red, not for mercury lamps

Filters in Olympus AX70 Provis Microscope

Olympus equivalents given, because that's how the cubes are labeled on the AX70 panel.

2025-4-14 Purchased from AVR Optics

BFP-A-BASIC-000 BrightLine Basic™ single-band filter set, optimized for Blue Fluorescent Protein and other like fluorophores (roughly equivalent to Olympus U-MWBV)

GFP-A-BASIC-000 BrightLine Basic™ single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores (roughly equivalent to Olympus U-MWB)

TXRED-4040C-000 BrightLine® single-band filter set, optimized for Texas Red and other like fluorophores (roughly equivalent to Olympus U-MNG)