Intraoral Catheter

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Method Description

Intraoral Catheterization and Infusions

Under isoflurane anesthesia, rats (n=16) were implanted with intraoral catheters made of PE-90 tubing that entered the mouth through the lateral check and were externalized on the dorsal surface between the scapulae, as described previously (Eckel and Ossenkopp, 1994). Intraoral catheters were flushed daily with water to maintain patency. For intraoral infusions, rats were weighed and placed in a glass aquarium subdivided into 4 individual compartments by Plexiglas sheets. Syringe pumps infused fluid into the mouth at a rate of 1 ml/min over 6 min. The rats were observed during the intraoral infusions; the time at which the rats first allowed the sucrose to passively drip from their mouths was recorded to the nearest second as the latency to drip. After the infusion, rats and any feces were weighed again as a measure of consumption, and rats were returned to their home cages.

To habituate rats to intraoral infusions, four days after surgery the rats were removed from their home cages and given intraoral infusions of deionized distilled water (6 ml over 6 min) on three consecutive days. After the third water infusion, rats were overnight food-deprived. Rats (n=8) received an infusion of 5% sucrose (6 ml/6 min) followed 30 minutes later by an injection of LiCl (0.15M, 12 ml/kg, i.p.). A separate group of rats received an intraoral infusion of 5% sucrose (6 ml/6 min) followed 30 min later by an injection of NaCl (0.15M, 12 ml/kg, i.p.). Food was returned one hour after the injections. On the final test day, rats recieved an intraoral infusion of 5% sucrose (6 ml / 6 min).

Materials

Intraoral catheter:

PE90 .034" ID .050" OD x 6 inches in length

BD Intramedic #427420, ordered from VWR #63018-704 for 10' at $50

Anchor washer for mouth:

PTFE Flat washer Ansi #0 .068 ID .188 OD .025 thick

Amazon B000FMYC7K $5.61/package of 10

Retaining Washer on back:

instead of using a teflon washer as indicated, we make our own that have smaller ID and larger OD

punch out disc of thin plastic using standard office hole punch; melt hole with hot 21 gauge needle

Silastic sleeve:

.058" ID .077" OD silicone tubing, approx. 1 inch in length

(not sure of current source)

Sliastic adhesive:

A-100: Medical Silicone Adhesive 2 oz/57g tube (formerly Corning Silastic Medical Adhesive Silicone Type A)

Purchased from Factor II, inc #A-100 $31.95

(required considerable paperwork, including affidavit that we wouldn't use in humans.)