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Line 91: − + − − ==Reagents and Solutions== − − ''prices as of 2011-11'' − − Denhardt's Solution 50x, 100 ml, Invitrogen # 750018 $122 − :(made from PVP (polyvinyl pyrrolidone) 40 & Ficoll Type 400)DEPC (diethylpyrocarbonate), Sigma #D-5758-50 ml $203 − − Dextran Sulfate sodium salt, Sigma #D-8906-50 g, $143 − − DTT (dithiothreitol) (aka Cleland's reagent) Ultrapure, 5 g, Invitrogen #15508013 $102.00 − − Formamide Ultrapure, 500g bottle, Invitrogen #15515026 $97 − − Salmon Sperm DNA 10 mg/ml, 10 x 1 ml tubes, Invitrogen #AM9680 $198 − SSC Buffer 20x Ultrapure, 1L bottle, Invitrogen #15557-044 $34
In Situ Hybridization (view source)
Revision as of 15:18, 22 November 2011
, 15:18, 22 November 2011separated reagents and solutions
==Protocol==
==Protocol==
==Reagents==
''prices as of 2011-11''
Denhardt's Solution 50x, 100 ml, Invitrogen # 750018 $122
:(made from PVP (polyvinyl pyrrolidone) 40 & Ficoll Type 400)DEPC (diethylpyrocarbonate), Sigma #D-5758-50 ml $203
Dextran Sulfate sodium salt, Sigma #D-8906-50 g, $143
DTT (dithiothreitol) (aka Cleland's reagent) Ultrapure, 5 g, Invitrogen #15508013 $102.00
Formamide Ultrapure, 500g bottle, Invitrogen #15515026 $97
Salmon Sperm DNA 10 mg/ml, 10 x 1 ml tubes, Invitrogen #AM9680 $198
SSC Buffer 20x Ultrapure, 1L bottle, Invitrogen #15557-044 $34
== Solutions==
'''DEPC-Treated Water'''
'''DEPC-Treated Water'''
*1 ml of DEPC per 1 L ddH2O
*1 ml of DEPC per 1 L ddH2O
'''DTT (dithiothreitol)'''
'''DTT (dithiothreitol)'''
Aka Cleland's reagent; prevents oxidation of thiol groups and breaks down disulfide bonds
Aka Cleland's reagent; prevents oxidation of thiol groups and breaks down disulfide bonds.
==Methods Section==
==Methods Section==