1,114
edits
Changes
Jump to navigation
Jump to search
m
In Situ Hybridization (view source)
Revision as of 15:18, 22 November 2011
, 15:18, 22 November 2011separated reagents and solutions
Line 5:
Line 5:
−==Reagents and Solutions==
−
−''prices as of 2011-11''
−
−Denhardt's Solution 50x, 100 ml, Invitrogen # 750018 $122
−:(made from PVP (polyvinyl pyrrolidone) 40 & Ficoll Type 400)DEPC (diethylpyrocarbonate), Sigma #D-5758-50 ml $203
−
−Dextran Sulfate sodium salt, Sigma #D-8906-50 g, $143
−
−DTT (dithiothreitol) (aka Cleland's reagent) Ultrapure, 5 g, Invitrogen #15508013 $102.00
−
−Formamide Ultrapure, 500g bottle, Invitrogen #15515026 $97
−
−Salmon Sperm DNA 10 mg/ml, 10 x 1 ml tubes, Invitrogen #AM9680 $198
−SSC Buffer 20x Ultrapure, 1L bottle, Invitrogen #15557-044 $34
==Protocol==
==Protocol==
+
+
+==Reagents==
+
+''prices as of 2011-11''
+
+Denhardt's Solution 50x, 100 ml, Invitrogen # 750018 $122
+:(made from PVP (polyvinyl pyrrolidone) 40 & Ficoll Type 400)DEPC (diethylpyrocarbonate), Sigma #D-5758-50 ml $203
+
+Dextran Sulfate sodium salt, Sigma #D-8906-50 g, $143
+
+DTT (dithiothreitol) (aka Cleland's reagent) Ultrapure, 5 g, Invitrogen #15508013 $102.00
+
+Formamide Ultrapure, 500g bottle, Invitrogen #15515026 $97
+
+Salmon Sperm DNA 10 mg/ml, 10 x 1 ml tubes, Invitrogen #AM9680 $198
+
+SSC Buffer 20x Ultrapure, 1L bottle, Invitrogen #15557-044 $34
+
+== Solutions==
'''DEPC-Treated Water'''
'''DEPC-Treated Water'''
*1 ml of DEPC per 1 L ddH2O
*1 ml of DEPC per 1 L ddH2O
Line 71:
Line 91:
'''DTT (dithiothreitol)'''
'''DTT (dithiothreitol)'''
−Aka Cleland's reagent; prevents oxidation of thiol groups and breaks down disulfide bonds
+Aka Cleland's reagent; prevents oxidation of thiol groups and breaks down disulfide bonds.
−==Methods Section==
==Methods Section==