Difference between revisions of "Fluorescent Microscopy"

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(Fix table to use tabs.)
(added some more)
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Neurotrace 435 455 blue
 
Neurotrace 435 455 blue
 
DAPI 358 461 blue
 
DAPI 358 461 blue
Fluorescein (FITC) 492 520 green
+
'''DyLight 405''' 400 421 blue
 +
Brilliant Violet 421 407 421 blue (expensive)
 +
'''GFP''' 425 500 green
 +
'''Fluorescein (FITC)''' 492 520 green
 +
YFP 500 535 yellow
 
Cyanine Cy3 550 570 orange
 
Cyanine Cy3 550 570 orange
Texas Red: 596 620 red
+
Alexa Fluor 568 577 602 red
Cyanine Cy5: 650 670 red
+
Texas Red 589 615 red
YFP 500 535 yellow
+
'''Alexa Fluor 594''' 591 614 red (sub for Texas Red)
GFP 425 500 green
+
Cyanine Cy5 650 670 red
 +
Alexa Fluor 647 red, not for mercury lamps
 
</tab>
 
</tab>

Revision as of 10:54, 5 February 2025

Here are the immunofluorescence filters that we probably need.

Probe Excitation (nm) Emission (nm) Color -
Neurotrace 435 455 blue
DAPI 358 461 blue
DyLight 405 400 421 blue
Brilliant Violet 421 407 421 blue (expensive)
GFP 425 500 green
Fluorescein (FITC) 492 520 green
YFP 500 535 yellow
Cyanine Cy3 550 570 orange
Alexa Fluor 568 577 602 red
Texas Red 589 615 red
Alexa Fluor 594 591 614 red (sub for Texas Red)
Cyanine Cy5 650 670 red
Alexa Fluor 647 red, not for mercury lamps