Difference between revisions of "Methylation-Sensitive Enzymes"
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Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site. | Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site. | ||
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Enzyme NEB link Site Cut Position Reaction Conditions | Enzyme NEB link Site Cut Position Reaction Conditions | ||
HpaII https://www.neb.com/products/r0171-hpaii CCgg 5’...C/CGG...3'<br>3’...GGC/C...5’ 1X NEBuffer 1, Incubate at 37°C. | HpaII https://www.neb.com/products/r0171-hpaii CCgg 5’...C/CGG...3'<br>3’...GGC/C...5’ 1X NEBuffer 1, Incubate at 37°C. | ||
Latest revision as of 14:40, 4 July 2022
Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site.
| Enzyme | NEB link | Site | Cut Position | Reaction Conditions |
|---|---|---|---|---|
| HpaII | https://www.neb.com/products/r0171-hpaii | CCgg | 5’...C/CGG...3' 3’...GGC/C...5’ |
1X NEBuffer 1, Incubate at 37°C. |
| MspI | https://www.neb.com/products/r0106-mspi | CCgg | 5’...C/CGG...3' 3’...GGC/C...5’ |
1X NEBuffer 4, Incubate at 37°C. |
| AciI | https://www.neb.com/products/r0551-aciip | CCgC | 5’...C/CGC...3' 3’...GGC/G...5’ |
1X NEBuffer 3, Incubate at 37°C. |
| HhaI | https://www.neb.com/products/r0139-hhai | gCgC | 5’...GCG/C...3' 3’...C/GCG...5’ |
1X NEBuffer 4, Supplemented with 100 μg/ml Bovine Serum Albumin, Incubate at 37°C. |