Fluorescent Microscopy
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Here are the immunofluorescence filters that we probably need.
Probe | Excitation (nm) | Emission (nm) | Color | - |
Neurotrace | 435 | 455 | blue | |
DAPI | 358 | 461 | blue | |
DyLight 405 | 400 | 421 | blue | |
Brilliant Violet 421 | 407 | 421 | blue (expensive) | |
GFP | 425 | 500 | green | |
Fluorescein (FITC) | 492 | 520 | green | |
YFP | 500 | 535 | yellow | |
Cyanine Cy3 | 550 | 570 | orange | |
Alexa Fluor 568 | 577 | 602 | red | |
Texas Red | 589 | 615 | red | |
Alexa Fluor 594 | 591 | 614 | red (sub for Texas Red) | |
Cyanine Cy5 | 650 | 670 | red | |
Alexa Fluor 647 | red, not for mercury lamps |