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Serine Racemase (view source)

Revision as of 11:56, 23 January 2009

560 bytes added ,  11:56, 23 January 2009
added some realtime probes
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:20-s at 58 °C for serine racemase
 
:20-s at 58 °C for serine racemase
 
:followed by a 20-s extension at 72  °C.
 
:followed by a 20-s extension at 72  °C.
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=Probes for real time PCR=
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Primer sequences taken from Yoshikawa et al. 2004,  PMID 15337321 (did not use BioRad system)
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Rat [actually mouse] serine racemase. Genbank Ascension # NM013761
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Forward (bases 784-803)
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:CCT GCA GTG ATA GCT GGA CA
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Backward (bases 1052-1033)
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:AAG CCA ATG CTG GAT TTG AC
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Primer sequences taken from Yoshikawa et al. 2006,  PMID 16973158 (did not use BioRad system)
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Rat serine racemase mRNA (accession number NM198757)
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forward primer 664 - 683
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:ATT GCA AGA AAC TGG CCA TC
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reverse primer, 958-939
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:TCA GCA GCG TAC ACC TTC AC
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