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added some realtime probes
:20-s at 58 °C for serine racemase
:20-s at 58 °C for serine racemase
:followed by a 20-s extension at 72 °C.
:followed by a 20-s extension at 72 °C.
=Probes for real time PCR=
Primer sequences taken from Yoshikawa et al. 2004, PMID 15337321 (did not use BioRad system)
Rat [actually mouse] serine racemase. Genbank Ascension # NM013761
Forward (bases 784-803)
:CCT GCA GTG ATA GCT GGA CA
Backward (bases 1052-1033)
:AAG CCA ATG CTG GAT TTG AC
Primer sequences taken from Yoshikawa et al. 2006, PMID 16973158 (did not use BioRad system)
Rat serine racemase mRNA (accession number NM198757)
forward primer 664 - 683
:ATT GCA AGA AAC TGG CCA TC
reverse primer, 958-939
:TCA GCA GCG TAC ACC TTC AC