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Serotonin Genes (view source)

Revision as of 15:23, 11 February 2009

272 bytes added ,  15:23, 11 February 2009
fixed TPH2 PCR products
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for in situ hybridization
 
for in situ hybridization
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product size:  bp, range & ascension number?
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product size:   
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:TPH2for: 5’ -GAAGTTGGCGGGCTGGTGAGA–3’
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495 bp product corresponding to bases 319-813 of AY098915 (from Malek PMID 16115212).
 +
502 bp product corresponding to bases 312-814 of NM173839 (based on our lookup)
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''(Hasn't been sequenced by us yet.)''
   −
:TPH2rev: 5’ -TCGGCAAGCATGAGTGGGGTAGA–3’
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:TPH2for  base 312-334:
   −
Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992
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::5’ -GAAGTTGGCGGGCTGGTGAGA–3’
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:TPH2rev:bases 791-814
    +
::5’ -TCGGCAAGCATGAGTGGGGTAGA–3’
 +
 +
 +
Reference:
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Malek ZS, Dardente H, Pevet P, Raison S. Tissue-specific expression of tryptophan hydroxylase mRNAs in the rat midbrain: anatomical evidence and daily profiles. Eur J Neurosci. 2005 Aug;22(4):895-901. PMID 16115212
    
==Tryptophan Hydroxylase 2 polymorphisms==
 
==Tryptophan Hydroxylase 2 polymorphisms==
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TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’
 
TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’
 +
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Mouse accession number: NM_173391
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Rat accession Number: NM_173839
    
Reference:  Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473
 
Reference:  Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473
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