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Line 40:
Line 40: − + − :TPH2for: 5’ -GAAGTTGGCGGGCTGGTGAGA–3’ + + + − + − Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992+ + + + + + + Line 67:
Line 75: + + +
fixed TPH2 PCR products
for in situ hybridization
for in situ hybridization
product size: bp, range & ascension number?
product size:
495 bp product corresponding to bases 319-813 of AY098915 (from Malek PMID 16115212).
502 bp product corresponding to bases 312-814 of NM173839 (based on our lookup)
''(Hasn't been sequenced by us yet.)''
:TPH2rev: 5’ -TCGGCAAGCATGAGTGGGGTAGA–3’
:TPH2for base 312-334:
::5’ -GAAGTTGGCGGGCTGGTGAGA–3’
:TPH2rev:bases 791-814
::5’ -TCGGCAAGCATGAGTGGGGTAGA–3’
Reference:
Malek ZS, Dardente H, Pevet P, Raison S. Tissue-specific expression of tryptophan hydroxylase mRNAs in the rat midbrain: anatomical evidence and daily profiles. Eur J Neurosci. 2005 Aug;22(4):895-901. PMID 16115212
==Tryptophan Hydroxylase 2 polymorphisms==
==Tryptophan Hydroxylase 2 polymorphisms==
TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’
TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’
Mouse accession number: NM_173391
Rat accession Number: NM_173839
Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473
Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473