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TN Unconditioned Aversions in 14-3-3 Transgenics
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Revision as of 12:09, 20 March 2012
97 bytes added
,
12:09, 20 March 2012
revised quinine sulfate
Line 2:
Line 2:
Tests of unconditioned aversions to bitter, salt, or sour tastants
Tests of unconditioned aversions to bitter, salt, or sour tastants
+
+
==Quinine Sulfate==
30 micromolar quinine sulfate (MW 391.47)
30 micromolar quinine sulfate (MW 391.47)
−
1000x
=
30
mM quinine sulfate = 0.
03
* 392 g / liter =
11
.76 g / liter =
5
.
88g
/ 500 ml
+
100x
=
3
mM quinine sulfate = 0.
003
* 392 g / liter =
1
.76 g / liter =
0
.
588g
/ 500 ml
(note: solubility of quinine sulfate is only 0.12g/100ml)
−
2
ml of
1000x
+ 2 liters -> 30 micromolar quinine sulfate
+
20
ml of
100x
+ 2 liters -> 30 micromolar quinine sulfate
+
+
==NaCl==
600 mM NaCl (MW 60) = 36 g / liter = 72 g/ 2 liters
600 mM NaCl (MW 60) = 36 g / liter = 72 g/ 2 liters
+
+
==HCl==
0.01 M HCl (MW 36.5, comes in 10M solution)
0.01 M HCl (MW 36.5, comes in 10M solution)
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