TN Unconditioned Aversions in 14-3-3 Transgenics

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Continuation of TK CTA in 14-3-3 Transgenics and TL Stronger CTA in 14-3-3 Transgenics and TM Stronger NaCl CTA in 14-3-3 Transgenics.

Tests of unconditioned aversions to bitter, salt, or sour tastants

Quinine Sulfate

30 micromolar quinine sulfate (MW 391.47)

50x = 1.5 mM quinine sulfate = 0.0015 * 392 g / liter = 0.588 g / liter = 0.3g / 500 ml (note: solubility of quinine sulfate is only 0.12g/100ml)

40 ml of 50x + 2 liters -> 30 micromolar quinine sulfate

NaCl

600 mM NaCl (MW 60) = 36 g / liter = 72 g/ 2 liters

HCl

0.01 M HCl (MW 36.5, comes in 10M solution)

2 ml of 10M + 2 liters -> 0.01 M HCl