TK CTA in 14-3-3 Transgenics
Test of CTA in transgenic mice that overexpress R18 peptide, which blocks the ability of 14-3-3 to bind other proteins. Zhou's founder Line 132.
1-23-12 rev. 2012-2-7
Adam Kimbrough, Yuying Wu, Dr. Thomas A. Houpt, Dr. Yi Zhou
TK1-31 (1-8=mutant LiCl, 9-16= mutant NaCl, 17-24= wildtype LiCl, 25-31= wildtype NaCl)
8 wildtype females 7 wildtype males
8 mutant females 8 mutant males
LiCl mutant group TK 1-4 =mutant females TK 5-8 =mutant males
NaCl mutant group TK 9-12 = mutant females TK 13-16= mutant males
LiCl wildtype group TK 17-20 = wildtype females TK 21-24 = wildtype males
NaCl wildtype group TK 25-28 = wildtype females TK 29-31 = wildtype male
6 Days of water deprivation (3h, 3h, 1h, 1h, 30m, 30m -> conditioning 10m) with intakes recorded each day.
Conditioning Day: 2012-2-7
10 minute 0.125% saccharin access (2g/liter; 4g/2 liters)
10 minutes after saccharin access injection with 0.15M LiCl or NaCl (20 ml/kg-intermediate mouse dose)
6 days 24-h 2 bottle access to 0.125% saccharin and water with intakes recorded.
Experiment continued as TL Stronger CTA in 14-3-3 Transgenics