TL Stronger CTA in 14-3-3 Transgenics
Test of stronger CTA in transgenic mice that overexpress R18 peptide, which blocks the ability of 14-3-3 to bind other proteins.
This is a follow-up (using the same mice) to experiment TK CTA in 14-3-3 Transgenics. The dose of LiCl was increased to 40 ml/kg 0.15M i.p.
Adam Kimbrough, Yuying Wu, Dr. Thomas A. Houpt, Dr. Yi Zhou
TK1-31 (1-8=mutant prev LiCl, 9-16= mutant prev NaCl, 17-24= wildtype prev LiCl, 25-31= wildtype prev NaCl)
8 wildtype females 7 wildtype males
8 mutant females 8 mutant males
LiCl mutant group TK 1,2,9,10 =mutant females TK 5,6,13,14 =mutant males
NaCl mutant group TK 3,4,11, 12 = mutant females TK 7,8,15,16= mutant males
LiCl wildtype group TK 17,18,25,26 = wildtype females TK 21,22,29,30 = wildtype males
NaCl wildtype group TK 19,20,27,28 = wildtype females TK 23,24,NEW31 NEW32 = wildtype male
6 Days of water deprivation (3h, 3h, 1h, 1h, 30m, 30m -> conditioning 10m) with intakes recorded each day.
Conditioning Day: 2012-2-23
10 minute 8% Polycose access (80g/liter)
10 minutes after Polycose access, injection with 0.15M LiCl or NaCl (40 ml/kg-high mouse dose)
14 days 24-h 2 bottle access to 8% Polycose (50g/liter) and water with intakes recorded.