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added Endres reference
Trimethylthiazoline (TMT), aka 2,4,5-trimethylthiazoline, aka 2,5-dihydro-2,4,5-trimethylthiazoline is a constiuent of fox urine that is an innately aversive odor to rodents.
Trimethylthiazoline (TMT), aka 2,4,5-trimethylthiazoline, aka 2,5-dihydro-2,4,5-trimethylthiazoline is a constiuent of fox urine that is an innately aversive odor to rodents.
C6H11NS, MW 129.22 PubChem CID 263626 CAS 189302-32-7
C6H11NS, MW 129.22, predicted (ACD labs) density 1.106 g/ml, [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=263626 PubChem CID 263626], CAS 189302-32-7
''NOT the same as: 2,4,5-Trimethylthiazole, amother predator odor.''
''NOT the same as: 2,4,5-Trimethylthiazole, another predator odor.''
[http://contech-inc.com ConTech Enterprises] (formerly Phero-Tech), TMT $150.00 1g
[http://contech-inc.com ConTech Enterprises] (formerly Phero-Tech), TMT $150.00 1g
==Notes on Use in Rodent Odor Aversion experiments==
==Notes on Use in Rodent Odor Aversion experiments==
As of April 2013, I suggest using 5 ul of 50% TMT (from Buron 2007)
As of April 2013, I suggest using 5 ul of 50% TMT (in mice from Buron 2007 PMID 17907837 )
or
5 ul of 1% TMT (in rats from Endres 2009 PMID 19617424) --''I think it is a 1% solution: Endres refers to "5 μl 10–1 % vol. TMT" as 3.87μmol. which assuming a density for TMT of 1 g/cm<super>3</super>, would be 5 ul of 1% (confusingly, 10-1 % = 0.1% is probably a typo; 1% = 10-0%).)''
We used TMT (Phero tech, Delta, BC, CANADA) as the predator odor, and rose oil (rose; ChromaDex, Irvine, CA, USA) was used for odor stimulation with and without TMT. Mice were placed singly in small cages without any floor chips. Following habituation for 5–10 min, a small piece (a quadrant of What- man no. 2 filter paper, which is 9 cm in diameter) of filter paper (Whatman Japan, Tokyo, Japan) was put in the cage, and 0.2 ml of odor solution was dropped on it. Different groups of animals were exposed to either filtered DDW for no-odor control (n = 12), 1.2 mM TMT alone (n = 18), 0.1% v/v rose alone (n = 18), or a combination of TMT and rose (n=18).
We used TMT (Phero tech, Delta, BC, CANADA) as the predator odor, and rose oil (rose; ChromaDex, Irvine, CA, USA) was used for odor stimulation with and without TMT. Mice were placed singly in small cages without any floor chips. Following habituation for 5–10 min, a small piece (a quadrant of What- man no. 2 filter paper, which is 9 cm in diameter) of filter paper (Whatman Japan, Tokyo, Japan) was put in the cage, and 0.2 ml of odor solution was dropped on it. Different groups of animals were exposed to either filtered DDW for no-odor control (n = 12), 1.2 mM TMT alone (n = 18), 0.1% v/v rose alone (n = 18), or a combination of TMT and rose (n=18).
Buron, 2007
Buron, 2007 PMID 17907837
In mice: Both ends of the corridor contained a watch glass with a filter paper soaked with either 5 ul of TMT (pure, diluted at 50% or 10%), or distilled water (control space), or 3 g of fox feces. [pure and 50% equally effacious at inducing avoidance; 10% significant but not very large avoidance]
In mice: Both ends of the corridor contained a watch glass with a filter paper soaked with either 5 ul of TMT (pure, diluted at 50% or 10%), or distilled water (control space), or 3 g of fox feces. [pure and 50% equally effacious at inducing avoidance; 10% significant but not very large avoidance]