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Cleavage by beta-galactosidase yields a dihalogen-substituted indoxyl, 5-bromo-4-chloro-3-indoxyl:
 
Cleavage by beta-galactosidase yields a dihalogen-substituted indoxyl, 5-bromo-4-chloro-3-indoxyl:
    
Oxidation in the presence of an electron acceptor yields a relatively insoluble indigoid dye:
 
Oxidation in the presence of an electron acceptor yields a relatively insoluble indigoid dye:
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=Oxidants used in histochemistry=
 
=Oxidants used in histochemistry=