45
edits
Changes
added agenda
Line 10:
Line 10:
=Agenda=
Rats typically display lying-on-belly (LOB) behavior in response to LiCl-induced malaise. The time to onset of LOB behavior has been shown to decrease as the dose of LiCl increases (2), so the time to onset of LOB can be used to infer the magnitude of LiCl-induced malaise. This experiment aims to determine if sodium selenate pretreatment weakens the toxic effects of LiCl by measuring the time to onset of LOB after LiCl (0.15M, 20 ml/kg, i.p.) administration in selenate-pretreated vs control rats.
Rats typically display lying-on-belly (LOB) behavior in response to LiCl-induced malaise. The time to onset of LOB behavior has been shown to decrease as the dose of LiCl increases (2), so the time to onset of LOB can be used to infer the magnitude of LiCl-induced malaise. This experiment aims to determine if sodium selenate pretreatment weakens the toxic effects of LiCl by measuring the time to onset of LOB after LiCl (0.15M, 20 ml/kg, i.p.) administration in selenate-pretreated vs control rats.
+
+=Agenda=
+
+* A 0.5mg/ml solution of sodium selenate in 0.15M NaCl was prepared the day before the experiment.
+
+* Male Sprague Dawley rats (n=6/group) were given selenate (1 ml/kg, i.p.) or NaCl (0.15M, 1 ml/kg, i.p.) two hours before receiving 6ml/kg, 12ml/kg or 20ml/kg injections of LiCl (0.15M, i.p.) or NaCl (0.15M, i.p.).
+
+* Immediately after the LiCl injections, rats were returned to their home cages and video recorded for 30-60 minutes.
+
+*Videos were scored for the time to onset of LOB for all rats.
{| class="wikitable"
{| class="wikitable"
Line 28:
Line 38:
| MBF06 || Sel/LiCl || Sel/NaCl || Sel/NaCl || NaCl/LiCl || NaCl/NaCl || Sel/LiCl
| MBF06 || Sel/LiCl || Sel/NaCl || Sel/NaCl || NaCl/LiCl || NaCl/NaCl || Sel/LiCl
|}
|}
−=Results=
=Results=