Changes

'''Day 0: Water removed''' (2023-9-1)

'''Day 0: Water removed''' (2023-9-12)

'''Days 1 - 11: Water Restriction''' (2023-8-27 - 2023-9-06)

'''Days 1 - 11: Water Restriction''' (2023-9-13 - 2023-9-23)

Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.  

Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.  

In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.

In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.

'''Day 12: Conditioning Day''' (2023-3-16)

'''Day 12: Conditioning Day''' (2023-9-24)

Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) after 10 min

Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) after 10 min

Groups:  

Groups: All mice receive CNO & LiCl injections

* JCC01-04: Female, vehicle

* JCE01-04: Female

* JCC05-08: Female, CNO

* JCE05-08: Female

* JCC09-12: Male, vehicle

* JCE09-12: Male

* JCC13-16: Male, CNO

* JCE13-16: Male

After injection, ad lib water returned overnight.

After injection, ad lib water returned overnight.

'''Day 13 - 24:  Preference Tests''' (2023-3-17 - 2023-3-24 )

'''Day 13 - 24:  Preference Tests''' (2023-9-25 - 2023-10-6 )

CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.

CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.