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JCE Inhibitory DREADD Functional Validation (view source)
Revision as of 12:21, 16 August 2023
, 12:21, 16 August 2023no edit summary
==Agenda==
==Agenda==
Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)
Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.
Pre Treatment 2 weeks before starting water restriction
Pre Treatment (8/18/23) 2 weeks before starting water restriction
4 groups:
4 groups:
3. Isotonic saline and 4-OHT, N=6
3. Isotonic saline and 4-OHT, N=6
4. Isotonic saline and sesame oil, N=4
4. Isotonic saline and sesame oil, N=5
'''Day 0: Water removed''' (2023-8-26)
'''Day 0: Water removed''' (2023-9-1)