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FosCreER DREADD PCR (view source)

Revision as of 12:03, 24 July 2024

339 bytes added ,  24 July
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{{lowercase title}}
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=Cycling Protocol=
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The below cycling protocol works for both DREADDs and the iCreER (TRAP2) allele.
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# 95.0° for 2 min
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# 95.0° for 20 sec
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# 65.0° for 15 sec, -0.5° per cycle
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# 72.0° for 10 sec
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# Goto 2, 10 times
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# 95.0° for 15 sec
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# 60.0° for 15 sec
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# 72.0° for 10 sec
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# Goto 6, 30 times
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# 72.0° for 5 min
     
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