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| + | {{lowercase title}} |
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| + | =Cycling Protocol= |
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| + | The below cycling protocol works for both DREADDs and the iCreER (TRAP2) allele. |
| + | # 95.0° for 2 min |
| + | # 95.0° for 20 sec |
| + | # 65.0° for 15 sec, -0.5° per cycle |
| + | # 72.0° for 10 sec |
| + | # Goto 2, 10 times |
| + | # 95.0° for 15 sec |
| + | # 60.0° for 15 sec |
| + | # 72.0° for 10 sec |
| + | # Goto 6, 30 times |
| + | # 72.0° for 5 min |
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