1,158
edits
Changes
added pH details
* 0.3 M TrisAmino
* 0.3 M TrisAmino
36.34g / liter
36.34g / liter, autoclave, pH to 7.4 with 38% HCl
* 0.15 M LiOH
* 0.15 M LiOH
3.6g / liter
3.6g / liter (no need to autoclave)
* 0.15 M LiCl
* 0.15 M LiCl
6.45g / liter
6.45g / liter, autoclave
* LiOr = 75mM LiOrotate, 150mM TrisAmino
* LiOr = 75mM LiOrotate, 150mM TrisAmino
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiOH, 0.47g orotic acid
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiOH, 0.47g orotic acid, pH to 7.4 with LiOH, filter
* LiCl = 75mM LiCl, 150mM TrisAmino
* LiCl = 75mM LiCl, 150mM TrisAmino
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiCl
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiCl, pH to 7.4 with LiOH, filter
* NaCl = 75mM NaCl, 150 mM TrisAmino
* NaCl = 75mM NaCl, 150 mM TrisAmino
20 ml of 0.3M TrisAmino, 20 ml of 0.15M NaCl
20 ml of 0.3M TrisAmino, 20 ml of 0.15M NaCl, pH to 7.4 with NaOH, filter
Ad lib water returned after final injection overnight.