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Line 3: − Test if lithium orotate (LiOr) induces a conditioned taste aversion comparable to LiCl, with a lower dose than SNA experiment+ Line 63:
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added Li and Na injection prep
Continuation of [[SNA Lithium Orotate CTA]].
Continuation of [[SNA Lithium Orotate CTA]].
Test if lithium orotate (LiOr) induces a conditioned taste aversion comparable to LiCl, with a lower dose than SNA experiment
Single pairing of saccharin intake (15 min) after 15-min delay with isomolar doses of LiCl and LiOr to see if LiOr is more potent.
Single pairing of saccharin intake (15 min) after 15-min delay with isomolar doses of LiCl and LiOr to see if LiOr is more potent.
* 75 mM LiOr, 6 ml/kg ip. (SNB03,06,09,12,15,16)
* 75 mM LiOr, 6 ml/kg ip. (SNB03,06,09,12,15,16)
all injections made isotonic with TrisAmino
all injections made isotonic with TrisAmino:
* 0.3 M TrisAmino
36.34g / liter
* 0.15 M LiOH
3.6g / liter
* 0.15 M LiCl
6.45g / liter
* LiOr = 75mM LiOrotate, 150mM TrisAmino
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiOH, 0.47g orotic acid
* LiCl = 75mM LiCl, 150mM TrisAmino
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiCl
* NaCl = 75mM NaCl, 150 mM TrisAmino
20 ml of 0.3M TrisAmino, 20 ml of 0.15M NaCl
ad lib water returned after final injection overnight.
ad lib water returned after final injection overnight.