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Continuation of [[SNA Lithium Orotate CTA]] and [[SNB Lithium Orotate CTA 2]] and [[SNC Non-Contingent Lithium Orotate]]
Test if OAT inhibitor naringin and UMPS inhibitor 6-azauracil decrease effectiveness of lithium orotate (LiOr) in inducing a conditioned taste aversion.
Inject narinign and 6-azauracil 30 min before single pairing of saccharin intake (15 min) after 15-min delay with LiOr.
naringin https://www.sigmaaldrich.com/US/en/product/sigma/71162 Sigma 71162-35g] $50.78: organic anion transporting polypeptide 1A2 (OATP1A2) inhibitor (see Pacholko 2023)
6-azauracil [https://www.sigmaaldrich.com/US/en/product/sigma/a1757 Sigma A1757-5g] $99.90: UMPS inhibitor (see Pacholko 2023)
{{Infobox experiment|align=right
| Name = Lithium Orotate Inhibitors
| project = Taste Aversion
| code = SND
| protocol = 20220000006
| funding = Chair's Fund
| investigators = Nabi, Houpt
| species = rat
| date = 2026-6-2
| type = CTA
| drugs = lithium orotate, lithium chloride
| data = https://houptlab.org/bartab/expt/?id=SND
}}
== Data ==
[https://houptlab.org/bartab/expt/?id=SND Raw Data Link]
== People ==
Soheila Nabi
==CTA Conditioning==
===Agenda===
Rats arrive (2026-5-26), n= 12 adult male Sprague Dawley rats (Charles River CD, d.o.b XXXXX), individually housed in BRF 169.
<br>
'''Day 0: Water removed''' (2026-6-2)
make labels for bottles.
<br>
'''Days 1 - 6: Water Restriction''' (2026-6-3 thru 2026-6-8)
Weigh rats daily. ad lib food.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 1500 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
<br>
'''Day 7: Conditioning Day''' (2026-6-9)
-30 min (~930): rats injected with naringin+6-azauracil (doses XXXX) or saline vehicle, XXXX ml/kg ip
0 min (~1000): all rats 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min (~1030): 6 rats injected with 75 mM LiOr, 6 ml/kg ip, or Tris-NaCl vehicle.
Thus, four groups:
- Veh-Veh SND01,05,09,13
- Veh-LiOr SND02,06,10,14
- NA-Veh SND03,07,11,15
- NA-LiOr SND04,08,12,16
Ad lib water returned after final injection overnight.
'''Days 8 - 22: Preference Tests''' (2026-6-10 through 2026-6-25)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 14 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]