SND Lithium Orotate Inhibitors
Continuation of SNA Lithium Orotate CTA and SNB Lithium Orotate CTA 2 and SNC Non-Contingent Lithium Orotate
Test if OAT inhibitor naringin and UMPS inhibitor 6-azauracil decrease effectiveness of lithium orotate (LiOr) in inducing a conditioned taste aversion.
Inject narinign and 6-azauracil 30 min before single pairing of saccharin intake (15 min) after 15-min delay with LiOr.
From Pacholko 2023 PMID 37356352:
naringin MW 580.53 Sigma 71162-35g $50.78: organic anion transporting polypeptide 1A2 (OATP1A2) inhibitor
6-azauracil MW 113.07 Sigma A1757-5g $99.90: UMPS inhibitor
For injection, combine 100 mg/kg naringin (17mM) + 30 mg/kg 6-azauracil (27mM) dissolved in saline/10% DMSO; in 10 ml/kg i.p. injection.
Note: Mix with saline on day of injection, otherwise will precipitate overnight.
- measure 400mg naringin + 120mg 6-azuracil;
- dissolve in 4ml DMSO with heavy vortexing
- slowly add 36 ml sterile injectable 0.9% saline, with frequent vortexing (-> clear light yellow solution)
- filter
Use 10% DMSO in injectable 0.9% saline as vehicle control.
| Lithium Orotate Inhibitors | |
|---|---|
| Taste Aversion | |
| Expt Code | SND |
| Start Date | 2026-6-2 |
| Investigators | Nabi, Houpt |
| Funding | Chair's Fund |
| Protocol | 20220000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | lithium orotate, lithium chloride |
| Data Link | https://houptlab.org/bartab/expt/?id=SND |
Data
People
Soheila Nabi
CTA Conditioning
Agenda
Rats arrive (2026-5-26), n=16 adult male Sprague Dawley rats (Charles River CD, d.o.b 2026-3-30), individually housed in BRF 169.
Day 0: Water removed (2026-6-2)
make labels for bottles.
Days 1 - 6: Water Restriction (2026-6-3 thru 2026-6-8)
Weigh rats daily. ad lib food.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 1000 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
Day 7: Conditioning Day (2026-6-9)
-30 min (~930): rats weighed and injected with naringin (100mg/kg) + 6-azauracil (30mg/kg) or saline vehicle, 10 ml/kg ip
0 min (~1000): all rats 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min (~1030): 6 rats injected with 75 mM LiOr, 6 ml/kg ip, or Tris-NaCl vehicle.
Thus, four groups:
- Veh-Veh (SND01,05,09,13)
- NA-Veh (SND02,06,10,14)
- Veh-LiOr (SND03,07,11,15)
- NA-LiOr (SND04,08,12,16)
Ad lib water returned after final injection overnight.
Days 8 - 22: Preference Tests (2026-6-10 through 2026-6-25)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 14 days.