1,216
edits
Changes
added reference and doses
Inject narinign and 6-azauracil 30 min before single pairing of saccharin intake (15 min) after 15-min delay with LiOr.
Inject narinign and 6-azauracil 30 min before single pairing of saccharin intake (15 min) after 15-min delay with LiOr.
From Pacholko 2023 PMID 37356352:
naringin [https://www.sigmaaldrich.com/US/en/product/sigma/71162 Sigma 71162-35g] $50.78: organic anion transporting polypeptide 1A2 (OATP1A2) inhibitor
6-azauracil [https://www.sigmaaldrich.com/US/en/product/sigma/a1757 Sigma A1757-5g] $99.90: UMPS inhibitor
For injection, combine 100 mg/kg naringin/30 mg/kg 6-azauracil dissolved in saline; in 10 ml/kg i.p. injection.
'''Day 7: Conditioning Day''' (2026-6-9)
'''Day 7: Conditioning Day''' (2026-6-9)
-30 min (~930): rats injected with naringin+6-azauracil (doses XXXX) or saline vehicle, XXXX ml/kg ip
-30 min (~930): rats injected with naringin (100mg/kg) + 6-azauracil (30mg/kg) or saline vehicle, 10 ml/kg ip
0 min (~1000): all rats 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
0 min (~1000): all rats 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)