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Weigh rats daily. ad lib food.
Weigh rats daily. ad lib food.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 1500 daily.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 1000 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
0 min (~1000): all rats 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
0 min (~1000): all rats 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min (~1030): 6 rats injected with 75 mM LiOr, 6 ml/kg ip, or Tris-NaCl vehicle.
30 min (~1030): 6 rats injected with 75 mM LiOr, 6 ml/kg ip, or Tris-NaCl vehicle.