Changes

* 0.3 M TrisAmino  

* 0.3 M TrisAmino  

  36.34g / liter, autoclave, pH to 7.4 with 38% HCl

  36.34g / liter, autoclave, pH to 8.0 with 38% HCl

* 0.15 M LiOH

* 0.15 M LiOH

* LiOr = 75mM LiOrotate, 150mM TrisAmino

* LiOr = 75mM LiOrotate, 150mM TrisAmino

  20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiOH, 0.47g orotic acid, pH to 7.4 with LiOH, filter

  20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiOH, 0.47g orotic acid, pH to 8 with 1N HCl, filter

* LiCl = 75mM LiCl, 150mM TrisAmino

* LiCl = 75mM LiCl, 150mM TrisAmino

  20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiCl, pH to 7.4 with LiOH, filter

  20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiCl, pH to 8 with 1N HCl, filter

* NaCl = 75mM NaCl, 150 mM TrisAmino

* NaCl = 75mM NaCl, 150 mM TrisAmino

  20 ml of 0.3M TrisAmino, 20 ml of 0.15M NaCl, pH to 7.4 with NaOH, filter

  20 ml of 0.3M TrisAmino, 20 ml of 0.15M NaCl, pH to 8 with 1N HCl, filter

Perfusion solutions:

Perfusion solutions: