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update with rat dob and some times
=== Perfusion Day ===
=== Perfusion Day (2026-10-31) ===
Rats injected with one of the following solutions (staggered in pairs by 15-20min):
Rats (n=9, d.o.ob 2026-7-2, arr. 2026-9-1) injected with one of the following solutions (staggered in pairs by 15-20min):
* 75 mM LiOr/Tris, 6 ml/kg ip. (SRA01, SRA04, SRA07)
* 75 mM LiOr/Tris, 6 ml/kg ip. (SRA01, SRA04, SRA07)
These are same doses used in [[SNB Lithium Orotate CTA 2]], which showed that LiOr had greater potency in CTA conditioning than LiCl.
These are same doses used in [[SNB Lithium Orotate CTA 2]], which showed that LiOr had greater potency in CTA conditioning than LiCl.
One hour after injection, rats are overdosed with sodium pentobarbital and transcardially perfused. Brains are dissected, and placed into 0.1M PB for storage, then transferred to 30% sucrose overnight before sectioning.
One hour after injection, rats are overdosed with sodium pentobarbital and transcardially perfused. Brains were dissected, postfixed for ~1 h, then transferred to 30% sucrose before sectioning.
* 0: inject SRA01,SRA02
* 0: inject SRA01,SRA02 (1015)
* 20: inject SRA03, SRA04
* 20: inject SRA03, SRA04
* 40: inject SRA05, SRA06
* 40: inject SRA05, SRA06
* 120: perfuse SRA07, SRA08
* 120: perfuse SRA07, SRA08
* 140: perfuse SRA09
* 140: perfuse SRA09
30% sucrose (1500)
=== c-Fos Immunohistochemistry ===
=== c-Fos Immunohistochemistry ===