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updated with actual pH of 8.0
* 0.3 M TrisAmino
* 0.3 M TrisAmino
36.34g / liter, autoclave, pH to 7.4 with 38% HCl
36.34g / liter, autoclave, pH to 8.0 with 38% HCl
* 0.15 M LiOH
* 0.15 M LiOH
* LiOr = 75mM LiOrotate, 150mM TrisAmino
* LiOr = 75mM LiOrotate, 150mM TrisAmino
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiOH, 0.47g orotic acid, pH to 7.4 with LiOH, filter
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiOH, 0.47g orotic acid, pH to 8 with 1N HCl, filter
* LiCl = 75mM LiCl, 150mM TrisAmino
* LiCl = 75mM LiCl, 150mM TrisAmino
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiCl, pH to 7.4 with LiOH, filter
20 ml of 0.3M TrisAmino, 20 ml of 0.15M LiCl, pH to 8 with 1N HCl, filter
* NaCl = 75mM NaCl, 150 mM TrisAmino
* NaCl = 75mM NaCl, 150 mM TrisAmino
20 ml of 0.3M TrisAmino, 20 ml of 0.15M NaCl, pH to 7.4 with NaOH, filter
20 ml of 0.3M TrisAmino, 20 ml of 0.15M NaCl, pH to 8 with 1N HCl, filter
Perfusion solutions:
Perfusion solutions: