Primers for Davenport's Real-Time PCR experiment

Primers were ordered 2009 March from Sigma Aldrich

Probe Abbr Sequence Tm Size Genbank Reference
NR1 RTNR1FOR GTTCTTCCGCTCAGGCTTTG 66.0 66 RNU11418, 2396-2461 Yamamoto et al. 2008 PMID 17957211
RTNR1REV AGGGAAACGTTCTGCTTCCA 65.8
Serine Racemase RTSERRACFOR ATTGCAAGAAACTGGCCATC 63.9 295 NM198757, 664-958 Yoshikawa et al., 2006 PMID 16973158
RTSERRACREV TCAGCAGCGTACACCTTCAC 64.1
GADPH RTGADPHFOR TGCACCACCAACTGCTTAG 62.5 201 DQ403053.1, 397-597 Uno et al., 2002 PMID 12068078
RTGADPHREV GGATGCAGGGATGATGTTC 63.0
Note: GADPHFOR and REV maybe mixed up . . . .

Primers for Real-Time PCR

Taken from Yamamoto et al. 2008 PMID 17957211 using Applied Biosystems

NR1

Forward primer	5'-GTTCTTCCGCTCAGGCTTTG-3'
Reverse primer	5'-AGGGAAACGTTCTGCTTCCA-3'
TaqMan probe	5'-FAM-CGGCATGCGCAAGGACAGCC-TAMRA-3'

NR2A

Forward primer	5'-AGCCCCCTTCGTCATCGTA-3'
Reverse primer	5'-GACAGGGCACCGTGTTCCT-3'
TaqMan probe	5'-FAM-AGGACATAGACCCCCTGACTGAGACCTGTG-TAMRA-3'

NR2B

Forward primer	5'-CCCCCAAGTTCTGGTTGGT-3'
Reverse primer	5'-TTTTGGGAACGAGCTTTGCT-3'
TaqMan probe	5'-FAM-TTGGCCGTCTTGGCCGTATCAGG-TAMRA-3'

NR2C

Forward primer	5'-GGGTGATGATGTTCGTGATGTG-3'
Reverse primer	5'-GTGAGGTTCTGGTTGTAGCTGACA-3'
TaqMan probe	5'-FAM-TCACGGTGGTTGCCATCACTGTCTTC-TAMRA-3'