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DME Sodium Butyrate and Flavor Conditioning 2 (view source)
Revision as of 11:34, 19 July 2020
, 11:34, 19 July 2020touch up
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
==Data==
==Data==
==Agenda==
==Agenda==
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n=18 adult male and female sprague-dawley rats (Charles River CD), individually housed in BRF 169.
n=18 adult male and female sprague-dawley rats (Charles River CD), individually housed in BRF 169.
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'''Days 0: Food and water removed.''' (July 10, 2020)
'''Days 0: Food and water removed.''' (July 10, 2020)
'''Days 1: Food and water restricted.''' (July 11 - July 17, 2020)
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'''Days 1-7: Food and water restricted.''' (July 11 - July 17, 2020)
Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight.
Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight.
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'''Day 9: Conditioning Day 1''' (July 18, 2020)
'''Day 8: Conditioning Day 1''' (July 18, 2020)
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 4% glucose (counterbalanced cherry, grape) at 1135am - 1155am
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 4% glucose (counterbalanced cherry, grape) at 1135am - 1155am
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'''Day 10: Conditioning Day 2''' (July 19, 2020)
'''Day 9: Conditioning Day 2''' (July 19, 2020)
0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape) at 1106-1136am
0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape) at 1106-1136am
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'''Days 11-17: 2 bottle preference tests''' (July 20 - 28, 2020)
'''Days 10-18: 2 bottle preference tests''' (July 20 - 28, 2020)
Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day.
Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day.
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'''Notes:'''
* Rat DME 16 did not drink from CS+/glucose on conditioning day 1
* CS- bottles not weighed in conditioning day 2, so estimated volumetrically from fill lines
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[[Category:Flavor Preference]]
[[Category:Flavor Preference]]