1,150
edits
Changes
Jump to navigation
Jump to search
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances preference learning
Replication of [DMC Sodium Butyrate and Flavor Conditioning] with following modifications:
1. To prevent formation of side preferencs, rats were trained with 2 choice water and 0.05% saccharin prior to conditioning
2. To prevent ceiling effect of preferences being too strong, CS+ used only 4% glucose
3. n=9 male, n=9 female, counterbalanced
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
==Data==
'''[https://houptlab.org/bartab/expt/?id=DME Raw Data Link]'''
==People==
Durba Mukherjee
==Agenda==
<br>
'''Rats arrive.''' (July 7, 2020)
n=18 adult male and female sprague-dawley rats (Charles River CD), individually housed in BRF 169.
'''Days 0: Food and water removed.''' (July 10, 2020)
'''Days 1: Food and water restricted.''' (July 11 - July 17, 2020)
Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight.
30 min water at 1100am daily, pellet ration at 1130am
<br>
'''Day 9: Conditioning Day 1''' (July 18, 2020)
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 4% glucose (counterbalanced cherry, grape) at 1135am - 1155am
30 min: remove CS+, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl, 1200am-1213am
Give daily pellet ration at 1215am
* Rats 1-9 : female, Rats 10-18: male
* Rats 1,2,5,6,9,10,13,14,17,18: Cherry CS+
* Rats 3,4,7,8,11,12,15,16: Grape CS+
* Odd rats: NaB injection; Even rats: NaCL injection
<br>
'''Day 10: Conditioning Day 2''' (July 19, 2020)
0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape) at 1106-1136am
''CS- bottles not weighed, so estimated volumetrically from fill lines.''
30 min: remove CS-, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl,
return food and water ad lib
* Rats 1-9 : female, Rats 10-18: male
* Rats 1,2,5,6,9,10,13,14,17,18: Grape CS-
* Rats 3,4,7,8,11,12,15,16: Cherry CS-.
* Odd rats: NaB injection; Even rats: NaCL injection.
<br>
'''Days 11-17: 2 bottle preference tests''' (July 20 - 28, 2020)
Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day.
<br>
<br>
[[Category:Flavor Preference]]
DME Sodium Butyrate and Flavor Conditioning 2 (view source)
Revision as of 10:29, 19 July 2020
, 10:29, 19 July 2020created and put in current dates
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances preference learning
Replication of [DMC Sodium Butyrate and Flavor Conditioning] with following modifications:
1. To prevent formation of side preferencs, rats were trained with 2 choice water and 0.05% saccharin prior to conditioning
2. To prevent ceiling effect of preferences being too strong, CS+ used only 4% glucose
3. n=9 male, n=9 female, counterbalanced
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
==Data==
'''[https://houptlab.org/bartab/expt/?id=DME Raw Data Link]'''
==People==
Durba Mukherjee
==Agenda==
<br>
'''Rats arrive.''' (July 7, 2020)
n=18 adult male and female sprague-dawley rats (Charles River CD), individually housed in BRF 169.
'''Days 0: Food and water removed.''' (July 10, 2020)
'''Days 1: Food and water restricted.''' (July 11 - July 17, 2020)
Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight.
30 min water at 1100am daily, pellet ration at 1130am
<br>
'''Day 9: Conditioning Day 1''' (July 18, 2020)
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 4% glucose (counterbalanced cherry, grape) at 1135am - 1155am
30 min: remove CS+, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl, 1200am-1213am
Give daily pellet ration at 1215am
* Rats 1-9 : female, Rats 10-18: male
* Rats 1,2,5,6,9,10,13,14,17,18: Cherry CS+
* Rats 3,4,7,8,11,12,15,16: Grape CS+
* Odd rats: NaB injection; Even rats: NaCL injection
<br>
'''Day 10: Conditioning Day 2''' (July 19, 2020)
0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape) at 1106-1136am
''CS- bottles not weighed, so estimated volumetrically from fill lines.''
30 min: remove CS-, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl,
return food and water ad lib
* Rats 1-9 : female, Rats 10-18: male
* Rats 1,2,5,6,9,10,13,14,17,18: Grape CS-
* Rats 3,4,7,8,11,12,15,16: Cherry CS-.
* Odd rats: NaB injection; Even rats: NaCL injection.
<br>
'''Days 11-17: 2 bottle preference tests''' (July 20 - 28, 2020)
Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day.
<br>
<br>
[[Category:Flavor Preference]]