Neurogenesis

Useful Markers

Ki67

marker for mitotic cells

a rabbit anti-rat Ki67 monoclonal antibody (1:750, Biomeda, Foster City, CA) From Corniola et al PMID 18778998

The nuclear protein Ki67 is expressed during all active phases of the cell cycle. Three weeks of dietary zinc deficiency reduced the number of Ki67-positive cells in the SGZ and GCL of the dentate, suggesting that zinc is required for neuronal precursor cell proliferation in vivo. This conclusion is supported by the finding that zinc restriction of human neuronal precursors in culture decreased the number of cells that entered S-phase, as evidenced by a reduction in BrdU labeling.

GAD67 (or was it 65?)

Look up references by Leo the olfactory fellow from NIH

Doublecortin

marker for migrating cells

Look up antibody used by Japanese Cre-Lox paper

NeuroTrace

Molecular Probes