AA Four Choice Preference
Adam Kimbrough, with help from Rachel Davenport
Start Date: Approx 9/8/08
Purpose: To establish a preference between 4 choices added to Kool-Aid and show that the preference can be conditioned.
Subjects: 8 rats individually caged
All solutions contain a base of 0.05% (1g/2l) Kool-Aid and 0.05% (1g/2l) saccharin
Kool-Aid flavors being used: Cherry, Grape, Lemonade, and Tropical Punch
Solutions are mixed with the base and one of the following:
- 0.15% (3g/2l) saccharin
- 8% (160g/2l) fructose
- 8% (160g/2l) glucose
- 8% (160g/2l) maltodextrin (polycose)
Experiment will run for 4 days using 4 bottles (1 of each solution) in the cages to establish preference.
Experiment will then run for 4 more days using 4 bottles each with Kool-Aid base solutions only to measure conditioning of preference.
- Remove the bottles from the cages
- Weigh the bottles to measure amount of liquid consumed
- Empty and rinse the bottles thoroughly
- Refill the bottles with the 4 solutions
- Weigh the bottles to record how much liquid is in each bottle
- Place bottles in random positions in the cage
- Check food level in cage (make sure they have enough)