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listed TPH2 primers
I am interested in 4 of the genes related to serotonin function in depression and food intake:
Pet-1
Serotonin Transporter
Tryptophan Hydroxylase 2
5HT2C Receptor
=PCR primers=
Tryptophan Hydroxylase 2 cDNA for in situ hybridization
product size: bp, range & ascension number?
TPH2for GAAGTTGGCGGGCTGGTGAGA 21 bases
TPH2rev TCGGCAAGCATGAGTGGGGTAGA 23 bases
Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992
Tryptophan Hydroxylase 2 polymorphism linked to aggression
Positive control, product size 523 bp
ctrlTPH2for: 5’ -tttgacccaaagacgacctgcttgca –3’
ctrlTPH2rev: 5’-tgcatgcttactagccaaccatgagaca-3’
1473C allele: 307bp
TPH2C1473: 5’-cagaatttcaatgctctgcgtgtggg-3’
1473G allele : 307 bp
TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’
Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473