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| − | I am interested in 4 of the genes related to serotonin function in depression and food intake: | + | I am interested in 4 of the genes related to serotonin function in depression and food intake: Pet-1, Serotonin Transporter (5HTT), Tryptophan Hydroxylase 2 (TPH2), and 5HT2C Receptor (5HT2CR) |
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| − | Pet-1
| + | = ISH probes= |
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| − | Serotonin Transporter
| + | ==Pet-1== |
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| − | Tryptophan Hydroxylase 2
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| − | 5HT2C Receptor | + | Reference: |
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| | + | ==Serotonin Transporter== |
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| | + | Reference: |
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| | + | ==Tryptophan Hydroxylase 2== |
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| | + | (see PCR primers below). |
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| | + | Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992 |
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| | + | ==5HT2C Receptor== |
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| | + | (see PCR primers below) |
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| | + | Reference: |
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| | Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992 | | Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992 |
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| | ==Tryptophan Hydroxylase 2 polymorphisms== | | ==Tryptophan Hydroxylase 2 polymorphisms== |
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| | Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473 | | Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473 |
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| | + | ==5HT2C Receptor cDNA== |
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| | + | for in situ hybridization |
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| | + | product size: bp, range & ascension number? |
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| | + | :5HT2Cfor: 5’ -CTATCCCTTACCTTCCTATTAC–3’ |
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| | + | :5HT2Crev: 5’ -TACCCTACTCTAGCATAGAAAC–3’ |
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| | + | Reference: ??? |