| Line 1: |
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| | =PET-1 Promoter= | | =PET-1 Promoter= |
| | | | |
| − | note at 1172 in the sequence below (i.e -828 from mRNA start) there appears to be an RAR element: '''gggtga'''gagaggca'''ccctctc'''
| + | According to TESS (reference below) |
| | + | |
| | + | There are the following potential RAR sites in the mouse PET-1 promoter: |
| | + | |
| | + | ''(Note: substract from 2000 to get upstream position)'' |
| | + | |
| | + | :RAR-alpha at 1061-1069 (La/Len = 1.53) |
| | + | :RAR-beta at 599-604 (1.69) |
| | + | :RAR-beta at 1596-1604 (1.71) |
| | + | :RAR-beta at 1926-1931 (1.71) |
| | + | |
| | + | :RXR-alpha/RXR-beta at 1595-1601 (2.0) |
| | + | :RXR-alpha/RXR-beta at 1926-1931 (2.0) |
| | + | |
| | + | Hi probability of ER-alpha binding (all La/Len = 1.9 - 2.0) at: |
| | + | :599-603 |
| | + | :1065-1069 |
| | + | :1597-1601 |
| | + | :1867-1871 |
| | + | :1926-1030 |
| | + | |
| | + | ER/SP-1 binding (all La/Len = 2.0) at: |
| | + | :990-994 |
| | + | :1243-1247 |
| | + | |
| | + | Found transcription factor binding sites using TESS: |
| | | | |
| | BLAST of mus FEV mRNA ( NM_153111) leads to 100% homology with mus chromosome 1 (AC104542.10) bases 38140 to 40385 | | BLAST of mus FEV mRNA ( NM_153111) leads to 100% homology with mus chromosome 1 (AC104542.10) bases 38140 to 40385 |
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| | Searched using TESS: Combined search query and defaults | | Searched using TESS: Combined search query and defaults |
| | | | |
| − | Chapter 5 of Wiley’s Current Protocols in Bioinformatics
| + | Schug, Jonathan, Using TESS to Predict Transcription Factor Binding Sites in DNA Sequence, Wiley’s Current Protocols in Bioinformatics, ed. Baxevanis, A. D. J. Wiley & sons, 2003. Chapter 5 |
| | | | |
| − | TESS: Transcription Element Search Software on the WWW', | + | TESS: Transcription Element Search Software on the WWW, |
| | Jonathan Schug and G. Christian Overton, | | Jonathan Schug and G. Christian Overton, |
| | Technical Report CBIL-TR-1997-1001-v0.0 | | Technical Report CBIL-TR-1997-1001-v0.0 |
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| | URL: http://www.cbil.upenn.edu/tess | | URL: http://www.cbil.upenn.edu/tess |
| − |
| |
| − | but it crashed...
| |